This month, I organized my experimental data and compiled histological imaging data in preparation for a potential future publication. I have been working on preparing figures for…

In February, I compiled and organized my existing data in preparation for figure drafting. At this stage, the manuscript may be structured in two main parts, and the first part…

I conducted exploratory singlecell transcriptome analyses to characterize cell populations in an in vitro differentiation setting, focusing on germlinelike and somaticlike…

In cell biology, a common approach has been to seed cells on 2D culture dishes. The method is simple and highly reproducible, but it is also a simplified model. As a result, it…

In Career Notes, I write reflections on how I think about my research, career, and life—capturing what happens as it happens, and what I learn along the way.

Last December, I explored transcriptomic differences between in vivo and in vitro somatic cells using dropletbased singlecell profiling. This analysis suggests that…

In humans and mice, oocytes—the precursor cells that can ultimately become eggs—are formed during fetal development, yet most of them disappear before reaching full maturity. In…

I have started to collect gene expression data as part of my ongoing work toward preparing a manuscript. Singlecell analysis requires substantial resources per experiment, so I…

This preprint by Elkouby et al. "Morphogenetic ovarian modules for oocyte production from germline stem cells" provides a comprehensive overview of ovarian module structures in…

I began organizing prior results toward a future manuscript submission. For figures, I used Illustrator; ChatGPT and similar tools assisted with routine lookups and workflow tips,…

Understanding organ development is one of biology's most fascinating questions. A new preprint "Ovarian development is driven by early spatiotemporal priming of the coelomic…

I have observed that prolonged culture periods are associated with increased cell attrition in my current system. Two nonexclusive explanations remain plausible: (1) stress…

Compound interest has recently become a widely discussed idea across domains. In The Almanack of Naval Ravikant, the importance of embedding compounding mechanisms into daily…

"Defining the cell and molecular origins of the primate ovarian reserve" <https://www.nature.com/articles/s41467025627020 In this study, the authors identify a PG1.5 state in…

I am exploring options to improve the culture system. Preliminary observations suggest that cell survival may be limited and apoptosis may occur under certain conditions; these…

In research, we form hypotheses, design experiments, and test them. These steps are essential in science, but they do not always translate well to everyday life or career…

Through longterm culture testing, several exploratory conditions are under evaluation, and data validation is ongoing. In coculture systems, recent reports and my preliminary…

There are various kinds of notetaking apps available today, such as Evernote, Notion, Apple Notes, and many others. However, it can be quite challenging to determine which one is…

Over the past month, I continued working on a longterm culture system aimed at modeling early germline development. This time, I noticed encouraging signs that suggest a possible…

As both a gynecologist and a researcher studying germ cells, I am continually amazed by these remarkable cells that form the foundation of human life. Germ cells eggs and sperm…

Developmentally, germ cells do not initially exist in the gonads but migrate through the body to reach them. After completing their migration, germ cells undergo mitosis within…

You can write in Markdown format, which makes it easy for AI to reference later, as it is a file format that AI can easily understand. In the future, as AI develops, it will…

One of the challenges in my current work has been balancing experimental complexity with observational clarity. In particular, some of the cell systems I’ve been working with made…